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Mixture/Dose Delivery System
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Mixture/Dose Delivery System
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Mixture/Dose Delivery System Mixture/Dose Delivery System[詳細(xì)]
2014-09-24 00:00
專利
瑞士IBS可編程蠕動(dòng)泵DOSE IT P910 瑞士IBS可編程蠕動(dòng)泵DOSEITP910技術(shù)參數(shù)蠕動(dòng)量0.1ml9’999ml流速0.6ml/min5L/min管道內(nèi)徑18mm管道壁厚1.5mm外型尺寸(長(zhǎng)x寬x高)203x210x191mm重量3.5kg接口2xRS232輸入電壓100240VAC50/60Hz訂購(gòu)信息儀器訂貨編號(hào)EDOSEITP910可編程蠕動(dòng)泵P910型，供貨范圍包括：171000■1硅膠管，內(nèi)徑4mm，長(zhǎng)2.5m■2吸液/分裝管，長(zhǎng)10cm，不銹鋼制，一端凹口■1吸液/分裝管，長(zhǎng)35cm，不銹鋼制，一端凹口■1管口夾■1移液管-硅膠管道連接頭■主管道轉(zhuǎn)換器配件訂貨編號(hào)：腳開關(guān)無(wú)需手動(dòng)操作，有連接電纜143200臺(tái)式開關(guān)無(wú)需手動(dòng)操作，有連接電纜171081具有分裝臂的曲頸瓶桿曲頸瓶桿直徑10mm171091擴(kuò)展泵頭雙泵頭裝配171090硅膠管組用于雙泵頭，直徑8mm,長(zhǎng)3.0m171088移液管-硅膠管道連接頭用于連接移液管和內(nèi)徑為2-6mm的硅膠管，171077[詳細(xì)]
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BD RevTet System使用說(shuō)明 BDRevTetSystem使用說(shuō)明pRevTet-On，pRevTet-Off，pRevTet-Off-IN，pRevTREIntroductionTheBDRevTet-OnTM&BDRevTet-OffTMSystemscombinetheadvantagesofretrovirus-mediatedgenetransferwiththeproventetracycline-regulatedcontrolofBDTet-OnTMandBDTet-OffTMGeneExpressionSystems.BDRevTetTMallowsthefastandefficientestablishmentofregulatedgeneexpressionsystemsinawidevarietyofcelltypes.EachRevTetSystemisacompleteretroviralgeneexpressionsystemcontainingaretroviralregulator（r）tTAvector,aretroviralresponsevector,acontrolvector,andapackagingcellline.LikeourBDRetro-XTMSystem（#631508）,theRevTetSystemscontaintheBDRetroPackTMPT67PackagingCellLine（#631510）forthesafe,efficientproductionofinfectious,replication-incompetentretrovirus.TheRevTetSystemsprovideapowerfulandconvenientapproachtosettingupthetetracycline（Tc）-inducible,high-levelgeneexpressionsystemsfirstdescribedbyGossenandBujard（1992）.Retroviral-mediatedtet-regulationTheTetSystemsarebasedontwoelementsfromthetetoperonoftheE.coliTn10transposontheTetrepressorprotein（TetR）andthetetoperatorDNAsequence（tetO）.ThegenetobeexpressedisclonedintothepRevTREresponsevectordownstreamofthetetracycline-responsiveelement（TRE）,whichcontainssevendirectrepeatsofthe42-bptetOsequenceandtheminimalimmediateearlypromoterofcytomegalovirus（PminCMV）.Thetwosystemsdifferintheirregulatoryvector（Figure1）.TheRevTet-OffSystemusesthepRevTet-OffVector,whichcontainsthetTAregulatoryelement,afusionofTetRandtheVP16activationdomain.TheBDRevTet-OnTMSystemusespRevTet-On,basedonthereversetTA（rtTA）.BothsystemsusethepRevTREresponsevector.AlltheRevTetVectorsarederivedfrompLNCX,aBDRetro-XTMvectorcapableofproducinghigh-titervirusintheappropriatepackagingcelllines.IntheRevTet-OffSystem,tTAbindstheTREandactivatestranscriptionintheabsenceofTcortheTcderivativedoxycycline（Dox）.Thus,asTcorDoxisaddedtotheculturemedium,transcriptionisturnedoffinadose-dependentmanner（Figure1）.Conversely,theRevTet-OnSystemallowsgeneexpressiontobeactivatedbytheadditionofDox.Thesetwocomplementarysystemsallowyoutochoosetheonethatbestsuitsyourneeds.BenefitsandapplicationsofTc-mediatedinductionInmostinduciblemammaliangeneexpressionsystems（e.g.,inductionbyheavymetals,steroidhormones,orheatshock）,inductionisnonspecificandexpressionlevelscannotbepreciselyregulated.Inaddition,thesesystemsaregenerallyleakyinthe“off”state,andtheinducingagentitselfmaybecytotoxicorhavepleiotropiceffectsonresults.Incontrast,regulationofgeneexpressionbytheheterologousbacterialcontrolelementsintheRevTetSystemsisveryspecific.Furthermore,thelevelsofTcorDoxrequiredforthefullrangeofgeneexpressionarenotcytotoxicandhavenosignificanteffectoncellproliferationoranimalgrowth,evenwithcontinuoustreatment（Bohletal.,1997;Mayfordetal.,1996）.TheuseofseparateregulatoryandresponsevectorsintheRevTetSystems質(zhì)粒圖譜:[詳細(xì)]
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Mixture/Dose Delivery System
brochure of gel documentation system
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