資料庫
儀器網(wǎng)>
資料庫>Design, synthesis and anti-tuberculosis activity of
Design, synthesis and anti-tuberculosis activity of
-
本文由 上海耐士科技有限公司 整理匯編
2024-09-29 03:25 633閱讀次數(shù)
文檔僅可預(yù)覽首頁內(nèi)容，請下載后查看全文信息！
-
立即下載
Design, synthesis and anti-tuberculosis activity of
登錄或新用戶注冊
請用手機(jī)微信掃描下方二維碼
快速登錄或注冊新賬號(hào)
微信掃碼，手機(jī)電腦聯(lián)動(dòng)
更多資料
-
Design, synthesis and anti-tuberculosis activity of- Design, synthesis and anti-tuberculosis activity of[詳細(xì)]
-
2024-09-29 03:25
專利
-
- Synthesis and xanthine oxidase inhibitory activity o
- Synthesis and xanthine oxidase inhibitory activity o[詳細(xì)]
-
2024-09-28 01:18
應(yīng)用文章
-
- Cytochrome Oxidase Activity
- CytochromeOxidaseActivityColorimetricAssayKitrev.04/13(Catalog#K287-100;100assays;Storeat-20°C)I.Introduction:CytochromecOxidase(EC1.9.3.1)orComplexIVisthefourthcomplexoftheElectronTransportChainlocatedinthemitochondrial(orbacterial)membrane.Itprovidesenergytothecellbycoup領(lǐng)electrontransportthroughtheCytochromecchainwiththeprocessofoxidativephosphorylation.ComplexIVcontains13differentsubunitsencodedbybothmitochondrialDNAandnuclearDNA.ItreceivesanelectronfromeachofthefourCytochromecmolecules,andtransfersittooneoxygenmolecule,convertingitintotwomoleculesofwater.Inthisprocess,italsobindstofourprotonmoleculesandtranslocatesthemacrossthemembranetoestablishelectrochemicalgradient,whichisutilizedforthesynthesisofATP.CytochromeOxidaseActivityAssayKitissimple,fastandhigh-throughputadaptable.Thisassaykitcanbeusedforpurifiedmitochondriaortissueextractscontainingmitochondria.TheactivityoftheenzymeisdeterminedcolorimetricallybyfollowingtheoxidationofreducedCytochromecasanabsorbancedecreaseat550nm.Theoverallreactionisasfollows:II.Application:FastandsimplemeasurementofCytochromeOxidaseenzymaticactivityin96-wellplateformat.Mitochondrialrespirationstudies,assemblyofthecomplexes,Mitochondriaoutermembraneintegrity.III.SampleType:PurifiedmitochondriaCells/TissueextractsIV.KitContents:V.UserSuppliedReagentsandEquipment:Multi-wellspectrophotometercapableofreadingabsorbance.Multi-channelpipetVI.StorageandHand領(lǐng):Storekitat-20°C,protectedfromlight.WarmAssayBuffertoroomtemperaturebeforeuse.KeepEnzymeDilutionBufferonice.Readtheentireprotocolbeforeperformingtheassay.VII.ReagentPreparationandStorageConditions:DTT:Aliquotandstoreat-20°C.Thawjustbeforeuse.Cytochromec:Reconstituteeachvialwith1mlofCytochromeOxidaseAssayBuffer.Mixbyvortexingtodissolvecompletely.Add5<lofDTTsolution.Mixwellandwaitfor15min.atroomtemperature.Keepthisworkingsolutionatroomtemperature.Afterassayiscompleted,aliquotandsaverestoftheCytochromecsolutionat-20°C.ThisisnowreducedformofCytochromec.VIII.ComplexIVActivityAssayProtocol:1.EfficiencyofReductionofCytochromec:Ina96-wellplate,mix20<lofreducedCytochromecwith100<lofCytochromeOxidaseAssayBuffer.PrepareaparallelwellasblankwithonlyAssayBuffer.ReadODat550nm.TheODat550nmofreducedCytochromecisbetween0.2-0.6.Ifnot,add5<lofDTT/mlofreconstitutedCytochromecandwaitfor15min.toreadagaintheOD.2.SamplePreparation:Isolatemitochondriafromculturedcells,yeastortissuesbyusingMitochondria/CytosolFractionationKit(BVcat.#K256)orYeastMitochondriaIsolationKit(K259-50)orusecellortissuelysate(BVcat.#1067).Therecommendedrangeofpurifiedmitochondriais0.5-5<gandtissueextractis1-60<gperreaction.Dilutethetestsamples,ifneededbyEnzymeDilutionBuffer.3.Cytochromecpreparation:Prepare1:6dilutionofCytochromecbyusingpre-warmedCytochromeOxidaseAssayBuffer(onepartofCytochromecto5partsofbuffer)inaseparatetubedependingonthenumberofassaysamplesandcontrols.Prepare120<lofdilutedCytochromecperreaction.4.ComplexIVActivityAssay:Beforethereaction,setthespectrophotometerat550nmonkineticprogramfor30-45minutesat30secinterval.Addthetestsamples(approx.volume5-10<l)toeachwellofa96-wellplate.Fornegativecontrol(Blank),addequalvolumeofEnzymeDilutionBuffer.Add120<lofthedilutedCytochromecfromStep3toeachsampleandcontrolusingamultichannelpipette.ShakeandimmediatelyreadandrecorddecreaseinODoveraperiodof30-45min.Note:Therateofthereactionisrelativetoacontrolornormalsample.Therateiscalculatedinlinearrange.5.Calculations:CalculaterateofthereactionbycalculatingchangeinOD:OD/minbyusingthemaximumlinearrate.Theoxidation[詳細(xì)]
-
2018-11-08 10:00
產(chǎn)品樣冊
-
- PR-water activity and dehydration
- PR-water activity and dehydration[詳細(xì)]
-
2024-09-20 13:35
報(bào)價(jià)單
-
- Amphiphilic Molecular Design as a Rational Strategy
- Amphiphilic Molecular Design as a Rational Strategy[詳細(xì)]
-
2008-11-14 00:00
應(yīng)用文章
-
- Design of a Portable Raman Sensor for Phosphorus
- Design of a Portable Raman Sensor for Phosphorus[詳細(xì)]
-
2008-02-15 00:00
報(bào)價(jià)單
-
- Combination of Orthogonal Array Design and Triangle
- Combination of Orthogonal Array Design and Triangle[詳細(xì)]
-
2024-09-20 13:34
報(bào)價(jià)單
-
- Glass sample holders - improved design
- Glass sample holders - improved design[詳細(xì)]
-
2024-09-29 11:09
操作手冊
-
- Respiration Activity MOnitoring System
- Respiration Activity MOnitoring System[詳細(xì)]
-
2008-05-05 00:00
產(chǎn)品樣冊
-
- PR-water activity and bakery products
- PR-water activity and bakery products[詳細(xì)]
-
2024-09-28 01:07
其它
-
- Microwave Synthesis for Everyone
- Microwave Synthesis for Everyone[詳細(xì)]
-
2006-01-05 00:00
報(bào)價(jià)單
-
- Microwave-Assisted Polymer Synthesis
- Microwave-Assisted Polymer Synthesis[詳細(xì)]
-
2006-04-30 00:00
應(yīng)用文章
-
- Microwave-assisted synthesis of allylic
- hehydrozirconationofalkyneswithzirconocenehydrochlorideandthedimethylzinc-mediatedadditionofalkenylzirconocenestodiphenylphosphinoyliminescanbegreatlyacceleratedwithmicrowaveirradiation,resultinginaconvenientandrapidone-potprocessforthepreparationofsyntheticallyusefulallylicamines.[詳細(xì)]
-
2018-08-17 10:00
產(chǎn)品樣冊
-
- Microwave-Assisted Stepwise synthesis
- Microwave-Assisted Stepwise synthesis[詳細(xì)]
-
2024-09-29 14:37
實(shí)驗(yàn)操作
-
- Foods vs. aw (water activity )
- Foods vs. aw (water activity )[詳細(xì)]
-
2024-09-27 23:46
選購指南
-
- Respiratory burst activity of Atlantic c
- Respiratory burst activity of Atlantic c[詳細(xì)]
-
2024-09-14 19:12
標(biāo)準(zhǔn)
-
- Structure and biological activity of pathogen-like s
- Structure and biological activity of pathogen-like s[詳細(xì)]
-
2024-09-20 13:30
產(chǎn)品樣冊
-
- Electrochemical synthesis and characterization of Ti
- Electrochemical synthesis and characterization of Ti[詳細(xì)]
-
2008-09-24 00:00
實(shí)驗(yàn)操作
-
- Synthesis and piezoelectric properties of nanocrysta
- Synthesis and piezoelectric properties of nanocrysta[詳細(xì)]
-
2007-08-29 00:00
報(bào)價(jià)單
-
- Synthesis and electro-polymerisation of a novel hete
- Synthesis and electro-polymerisation of a novel hete[詳細(xì)]
-
2015-02-27 00:00
操作手冊
Copyright 2004-2026 yiqi.com All Rights Reserved , 未經(jīng)書面授權(quán) , 頁面內(nèi)容不得以任何形式進(jìn)行復(fù)制
在線留言
滬公網(wǎng)安備 31011502008050號(hào)
參與評論
登錄后參與評論