This technical note describes kinetic fluorescence as an analytical technique to quantify non-fluorescent species. The technique is applied to the determination of thiamine (vitamin B1) in solution. Fluorescence intensity is monitored as thiamine converts to fluorescent thiochrome, and a relationship is established between the rate of increase of intensity and the concentration of thiamine. The technique is shown to have good sensitivity and selectivity. 高靈敏一體式熒光光譜儀-FluoroMax-4 模塊式熒光光譜儀-Fluorolog-3