• Genomics 89 (2007) 532540
ScienceDirect www.sciencedirect.com
www.elsevier.com/locate/ygeno
Method
An efficient method for producing an indexed, insertional-mutant library in rice
水稻插入突變體庫的有效建立方法康奈爾大學(xué)
在文中,將多個水稻葉片同時在SPEX Geno 2000高通量組織研磨儀內(nèi)進行研磨,研磨后的樣品直接進行DNA提取,可以一次獲得大規(guī)模的DNA樣本由于采用了高通量研磨儀,可以達到GX建立基因文庫的效果
Chengkun He, Moul Dey, Zhihong Lin, Faping Duan, Feng領(lǐng) Li, Ray Wu
Department of Molecular Biology and Genetics, Cornell University, 316 Biotechnology Building, Ithaca, NY 14853, USA
Received 9 August 2006; accepted 8 November 2006
Available online 16 January 2007
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