全文請訪問：http://www.spm.com.cn/papers/p39.pdf
Neurofilaments(NFs)are neuron-specific intermediate filaments.The NFs were isolated from bovine spinal cord by differential centrifugation.The NFs were detected with electron microscopy and scanning tunne領(lǐng) microscopy(STM).Under STM,two kinds of sidearm of NFs were revealed:one was short,the other was long.They were arrayed along the 10-nm width core filaments one by one.The intervals between two adjacent long sidearms or two short sidearms were 20-22nm,while those between two adjacent long and short sidearms were 10-11nm.It was proposed that the rod domain of NF triplet proteins was 3/4-staggered.The assembly properties of NF triplet proteins were also studied.Immuno-colloidal-gold labe領(lǐng) assay showed that NF-M and NF-H are able to co-assemble into long filaments with NF-L.NF-M and NF-H can also co-constitute into winding filaments. 全文請訪問：http://www.spm.com.cn/papers/p39.pdf 掃描探針顯微鏡(SPM/AFM/STM)