北京譜朋科技有限公司是一家服務于實驗室分析儀器領域內設備及備品配件的高科技公司公司秉承著專業(yè)誠信值得信賴的經營理念，致力于為工農業(yè)生產科研及商檢領域提供世界yi流的分析儀器產品和服務我們多年的經驗同積累，我們的專業(yè)和不斷地創(chuàng)新，我們的誠信和優(yōu)質服務，得到了各行業(yè)客戶的一致肯定和好評，為企業(yè)贏得了zhuo越商譽 客戶信賴的品牌供貨商是我們企業(yè)追求的目標，我們也時刻以此來嚴格要求自己代理及經營多項 The reversible phosphorylation of serine, threonine and tyrosine, is one of the most important post-translational modifications involved in various cellular functions. Identification of phosphorylation sites by mass spectrometry is challenging due to the low abundance of phosphopeptides and their limited ionization efficiency. Therefore, it is critical to selectively enrich the phosphopeptides prior to MS analysis. In this study we illustrate the use of a Phosphopeptide Enrichment Kit that consists of an affinity extraction µElution plate, and a unique chemical reagent under the trade name of Enhancer, which is shown to improve the selectivity of the affinity solid-phase extraction (SPE) towards phosphopeptides for highly complex bio­logical samples. Also, a vial of phosphopeptide standard is included in the kit as a control sample to validate the affinity extraction method. MALDI-TOF MS and nano-scale LC/MS were used to evaluate the per­formance of the affinity solid-phase extraction device. Recommended protocol for the affinity SPE µElution plate, and sample preparation for MS analysis are included in the application.