A simple method is proposed for copper, lead and manganese determination in deciduous teeth by graphite furnace atomic absorption spectrometry (GFAAS) using slurry samp領(lǐng) introduction and cryogenic sample preparation. Teeth samples were ground in a cryogenic mill in two steps: pre-coo領(lǐng) (5 min) and cryogenic grinding (2 min) in liquid nitrogen. After grinding, 90% of the sample particles were lower than 150 mm. The minimum mass necessary for slurry preparation as an indicator of sub-sample homogeneity was evaluated by weighting masses between 5 and 20 mg directly in autosampler cups, followed by addition of 1 mL of a solution containing 0.04% Triton1 X-100 and 0.2% v/v HNO3. Samples (20 mL) were sonicated during 20 s, before delivering into a WRh coated platform. Detection limits based on integrated absorbance were 34.0 ng g21 Pb, 7.4 ng g21 Mn and 18.0 ng g21 Cu for 2% m/v slurries. WRh permanent modifier permitted calibration against aqueous standards. The Certified Reference Material (H-5 animal bone) from the International Agency of Atomic Energy (IAEA) was analyzed to determine lead for method validation. For copper, lead and manganese, 12 human teeth samples were analyzed using the proposed method with calibration against aqueous solution and using a comparative Pd/Mg method with the same digested samples, and standard addition calibration, with no statistical difference at 95% level on applying the t-test. SPEX6770 全自動(dòng)冷凍研磨機(jī) SPEX6870 全自動(dòng)冷凍研磨機(jī) SPEX6970EFM 冷凍研磨機(jī)