資料庫
Northern雜交分析
-
本文由 上海士鋒生物科技有限公司 整理匯編
2015-11-17 00:00 925閱讀次數(shù)
文檔僅可預(yù)覽首頁內(nèi)容,請下載后查看全文信息!
-
立即下載
Northern雜交分析
登錄或新用戶注冊
請用手機微信掃描下方二維碼
快速登錄或注冊新賬號
微信掃碼,手機電腦聯(lián)動
更多資料
-
Northern雜交分析
- Northern雜交分析[詳細]
-
2015-11-17 00:00
操作手冊
-
SI30H雜交振蕩培養(yǎng)箱(分子雜交箱)產(chǎn)品樣冊
- SI30H雜交振蕩培養(yǎng)箱(分子雜交箱)產(chǎn)品樣冊[詳細]
-
2016-10-18 14:41
實驗操作
-
分子雜交探針種類
- 分子雜交探針種類[詳細]
-
2024-10-02 01:51
選購指南
-
英國 BIBBY STUART 雜交振蕩培養(yǎng)箱(分子雜交箱)SI30H產(chǎn)品樣冊
- 英國 BIBBY STUART 雜交振蕩培養(yǎng)箱(分子雜交箱)SI30H產(chǎn)品樣冊[詳細]
-
2016-10-20 09:34
期刊論文
-
分子雜交箱產(chǎn)品樣冊
- 分子雜交箱產(chǎn)品樣冊[詳細]
-
2024-09-20 22:51
選購指南
-
WIGGENS WO-10 雜交爐產(chǎn)品樣冊
- WIGGENS WO-10 雜交爐產(chǎn)品樣冊[詳細]
-
2016-10-19 18:06
其它
-
日本TAITEC雜交爐產(chǎn)品樣冊
- 日本TAITEC雜交爐產(chǎn)品樣冊[詳細]
-
2016-10-20 09:12
產(chǎn)品樣冊
-
大腸桿菌雜交及基因定位實驗
- 大腸桿菌雜交及基因定位實驗[詳細]
-
2016-01-12 00:00
專利
-
如何進行輻射性雜交的制圖
- 如何進行輻射性雜交的制圖[詳細]
-
2015-12-28 00:00
實驗操作
-
BlotCycler 全自動蛋白雜交儀視頻
- BlotCycler 全自動蛋白雜交儀視頻[詳細]
-
2024-09-28 00:19
產(chǎn)品樣冊
-
美國Stovall水浴雜交反應(yīng)器技術(shù)資料
- 美國Stovall水浴雜交反應(yīng)器技術(shù)資料[詳細]
-
2024-09-19 00:23
報價單
-
分子雜交箱/分子雜交儀產(chǎn)品樣冊
- 分子雜交箱/分子雜交儀產(chǎn)品樣冊[詳細]
-
2024-09-21 18:39
標(biāo)準(zhǔn)
-
四倍體期鼠胚胎干細胞雜交技術(shù)方法
- 四倍體期鼠胚胎干細胞雜交技術(shù)方法[詳細]
-
2014-09-19 00:00
選購指南
-
分子雜交方法檢測致病菌詳細說明
- 分子雜交方法檢測致病菌詳細說明[詳細]
-
2009-05-19 00:00
選購指南
-
Clontech pBridge酵母三雜交系統(tǒng)說明書
- ClontechpBridge酵母三雜交系統(tǒng)說明書載體描述:pBridgeexpressestwoproteins:aDNA-bindingdomainfusion,andanadditionalprotein.pBridgethusallowsestablishmentofthree-hybridsystemswhenusedincombinationwithanactivationdomainfusionvectorandyeaststrainsfromanyofClontech'sGAL4-basedtwo-hybridsystems,includingMatchmakerGold.ThisvectorgeneratesahybridproteinthatcontainsthesequencesfortheGAL4DNA-bindingdomain(DNA-BD)andthesequenceclonedintoMCSI.ThefusionproteinisexpressedinyeasthostcellsfromtheconstitutiveADH1promoter;transcriptionisterminatedattheADH1transcriptionterminationsignal.Thehybridproteinistargetedtotheyeastnucleusbynuclearlocalizationsequences(NLS)thatareanintrinsicpartoftheGAL4DNA-BD.AnadditionalgeneofinterestcanbeclonedintoMCSIIwhichislocateddownstreamofanHAepitopeandasecondNLS.TheresultingfusionproteinisconditionallyexpressedfromtheMet25promoterinresponsetomethioninelevelsinthemedium;i.e.,itisrepressedinthepresenceof1mMmethionineandexpressedintheabsenceofmethionine.質(zhì)粒圖譜:[詳細]
-
2018-09-29 10:03
產(chǎn)品樣冊
-
酵母單雜交系統(tǒng)Clontech pHis2說明書
- 酵母單雜交系統(tǒng)ClontechpHis2說明書DescriptionpHIS2isareportervectorthatcanbeusedinyeastone-hybridassaystoidentifyandcharacterizeDNA-bindingproteins.ThevectorwasspecificallydesignedforusewiththeBDMatchmakerOne-HybridLibraryConstruction&ScreeningKit(#K1617-1).ItcontainsaHIS3nutritionalreportergene,locateddownstreamofamultiplecloningsite(MCS)andtheminimalpromoteroftheHIS3locus(PminHIS3).Cis-actingDNAsequences,orDNAtargetelements,canbeinsertedintotheMCSandusedasbaitstoscreenGAL4AD/cDNAfusionlibrariesforproteinsthatinteractwiththetargetsequence.Aprotein-DNA(orone-hybrid)interactioncanbedetectedbyperformingtheassayinayeaststrainsuchasY187thatisauxotrophicforhistidine.Positiveone-hybridinteractionsdriveexpressionoftheHIS3reportergene,whichenablesthehostcelltogrowonhistidine-deficientmedia.Intheabsenceofactivation,theconstitutiveHIS3expressionfromPminHIS3isverylow.Duringlibraryscreening,theleakyexpressionofHIS3iscontrolledbyadding3-amino-1,2,4-triazole(3-AT)tothemedium.Theconcentrationof3-ATneededtofullysuppressleakyHIS3expressionmustbedeterminedempiricallyforeachDNAtargetelement.pHIS2canbemaintainedinbothyeastandbacteria.Itcontainsanautonomousreplicationsequence(ARS4)andTRP1nutritionalmarkerforreplicationandselectioninyeast(1,2);itcontainsaColE1originandakanamycinresistancegene(Kanr)forpropagationandselectioninE.coli.ThecentromericsequenceCEN6ensurespropersegregationoftheplasmidduringcelldivisioninyeast(1,2).UseTousepHIS2inaone-hybridassay,cloneoneormorecopiesofacis-actingDNAtargetsequenceintotheMCS.ThenintroducetheplasmidintocompetentyeastcellsusingthetransformationprotocolintheBDMatchmakerLibraryConstruction&ScreeningKitsUserManual(PT3529-1).IncontrasttotheoriginalBDMatchmakerOne-HybridSystem,thisreportervectordoesnotneedtobeintegratedintotheyeastgenome.Instead,itismaintainedasanepisomethroughouttheassay.InsertingyourtargetelementmayalterthelevelofbackgroundHIS3expression.Therefore,constructs首ldbetestedforbackground(leaky)HIS3expressionbeforeyoustartaone-hybridanalysis.BackgroundgrowthduetoleakyHIS3expressioniscontrolledbyadding3-ATtotheselectionmedium,asdescribedintheUserManual(PT3529-1).LocationofFeaturesMultiplecloningsites:141HIS3gene:152811FragmentcontainingtheHIS33'UTR&Terminationsequence:8121446TRP1gene:28553529FragmentcontainingtheColE1E.colioriginofreplication:41654615Kanamycin-resistancegene:56054811CEN6/ARS4sequences:62545737[詳細]
-
2018-09-29 10:03
產(chǎn)品樣冊
-
BIBBY 分子雜交箱HB-3D ,HB-1D產(chǎn)品樣冊
- BIBBY 分子雜交箱HB-3D ,HB-1D產(chǎn)品樣冊[詳細]
-
2016-10-20 09:34
報價單
-
VACUUBRAND RC 6化學(xué)雜交泵產(chǎn)品樣冊
- VACUUBRAND RC 6化學(xué)雜交泵產(chǎn)品樣冊[詳細]
-
2024-09-15 23:53
其它
-
Shake ‘n’ Stack 分子雜交箱產(chǎn)品樣冊
- Shake ‘n’ Stack 分子雜交箱產(chǎn)品樣冊[詳細]
-
2024-09-12 21:17
實驗操作
-
核酸分子雜交及其技術(shù)分類的簡單介紹
- 核酸分子雜交及其技術(shù)分類的簡單介紹[詳細]
-
2024-09-14 19:11
課件
Copyright 2004-2026 yiqi.com All Rights Reserved , 未經(jīng)書面授權(quán) , 頁面內(nèi)容不得以任何形式進行復(fù)制
參與評論
登錄后參與評論