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雞蛋中的氨基糖苷分析
Aminoglycosides in Eggs
Conditions
Column: ACE Excel 2 C18-PFP
Dimensions: 100 x 2.1 mm
Part Number: EXL-1010-1002U
Mobile Phase:
A: 20 mM HFBA in H2O/MeCN (98:2 v/v)
B: 20 mM HFBA in MeCN/H2O (98:2 v/v)
Time(mins) | %B | Curve |
0.0 | 5.0 | - |
2.0 | 15.0 | 6 |
4.5 | 19.0 | 6 |
5.5 | 19.5 | 8 |
6.0 | 22.0 | 6 |
7.0 | 35.0 | 6 |
9.0 | 48.0 | 8 |
9.5 | 5.0 | 6 |
Flow Rate: 0.4 mL/min
Temperature: 40 °C
Detection: Positive ESI MRM (transitions as shown)
Sample: Extraction at low pH, clean up with WCX SPE cartridge
Egg sample spiked at 100 μg/kg (CCα)
Key
GNT Gentamicin
TBM Tobramycin
KNM Kanamycin
DHS Dihydrostreptomycin
STR Streptomycin
HGB Higromycin-B
SPT Spectinomycin
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