This workflow involved the analysis of a highly complex protein digest and was dependent upon an LC/MS methodology that achieves the best possible peptide detection and analysis, in order to detect important, low-level protein components. In addition to this, many of the peaks used for quantification were small, but accurate comparisons were possible due to the large number of data points defining these peaks. The LTQ acquires five MS or MS/MS spectra/second, ensuring the best possible data for accurate analytical determinations such as these. Up-Regulation of CRP, as measured by a 2.2-fold increase in its raw MS signal (concentration) at T24 vs T0, is consistent with the observed general elevation of acutephase response factors of which it is a part. Future work will involve validation of unique putative markers that were also identified in this analysis.