薄層層析-紫外分光光度法測定香丹注射液中原兒茶醛的含量 薄層層析-紫外分光光度法測定香丹注射液中原兒茶醛的含量 作者：偶志紅，徐丹丹，郭薇 摘要 目的 建立薄層層析-紫外分光光度法測定香丹注射液中原兒茶醛的含量方法 以原兒茶醛為對照品，展開劑為二氯甲烷-乙酸乙酯-甲酸（850.8）分離得到原兒茶醛，采用紫外分光光度法，檢測波長281nm，測定原兒茶醛的含量結(jié)果 原兒茶醛在0.55～4.95g/ml 范圍內(nèi)線性關(guān)系良好，r=0.9998平均含量為0.174mg/ml，加樣回收率為97.3%～102.3%，RSD為1.6%結(jié)論 實驗結(jié)果表明，薄層層析-紫外分光光度法測定香丹注射液中原兒茶醛的含量，方法操作簡便，準確度高，精密度好，可作為樣品的檢測方法 關(guān)鍵詞 香丹注射液；原兒茶醛；薄層層析-紫外分光光度法；含量測定 Abstract Objective A TLC-UV method was developed to separate and determine protocatechuic aldehyde in injection saliae miltiorrhizae composita.Methods Contrasting with protocatechuic aldehyde， the amount of protocatechuic aldehyde in injection saliae miltiorrhizae composita have been determined by TLC-UV with methylene chloride-acetic acid acetic ester-formic acid（850.8）as the developing solvent and the detection wavelength at 281nm.Results The calibration carver was linear in the range of 0.55～4.95g /ml (r=0.9998)for protocatechuic aldehyde .The average amount is 0.174mg /ml.The recoveries of protocatechuic aldehyde are between 97.3% and 102.3%，and RSD% was not more than 2.0%.Conclusion The method is simple，rapid and reproducible for determination of protocatechuic aldehyde in injection saliae miltiorrhizae composita. Key words injection of saliae miltiorrhizae composita；protocatechuic aldehyde；TLC-UV；content determination 香丹注射液為棕色澄明液體，是由降香和丹參提取物配制而成，臨床用于ZL心絞痛 固相微萃取(SPME)