Introduction The multivariate SSE program can be used to analyze sets of CD data. A large number of samples can be automatically measured and efficiently analyzed using the high-throughput circular dichroism (HDX) system and multivariate SSE program. Keywords: Secondary structure estimation, Multiple samples, HTCD, HDX Sequence Optical constants are automatically calculated in the multivariate SSE program after imputing parameters JASCO圓二色光譜儀CD J-1500