· This paper discusses the identification of a peptide substrate for molecular chaperone Hsp40 Ydj1 using combination of phage display library screening and ITC · Molecular chaperone heat shock protein 40s (Hsp40s) play roles with Hsp70s to promote protein folding, assembly, translocation, degradation, and prion propagation. Hsp40 proteins interact with hydrophobic side chains of non-native polypeptides to prevent the non-native polypeptides from aggregating. Mechanism for binding is not understood 差示掃描式微量量熱儀MicroCal , USA