Extraction of nucleic acids from plants is a time consuming and labor intensive process. Two common methods of extraction are (i) grinding individual samples with mortar and pestle, and (ii) mechanical mincing. Unfortunately, these methods are prone to contamination, as the same tools are often reused sequentially for multiple preparations. This risk of contamination is often unacceptable, especially in studies using rare or valuable specimens, such as when breeding selection is critical (particularly with the advent of genetically engineered crops), or when intellectual property discovery or protection is involved. Here we compare cell isruption by Qiagen RNeasy with mortar and pestle to pressure cyc領(lǐng) technology PCT) for the extraction of RNA from corn sprouts, grape seeds, and grape skin. 利用壓力循環(huán)技術(shù)(PCT)從植物組織中提取RNA 從植物中提取核酸是一件非常耗時(shí)耗力的工作目前常見的提取方法有人工研磨和機(jī)械破碎兩種方法因?yàn)檠心スぞ吆推扑榈额^經(jīng)常反復(fù)使用，所以這兩種方法非常容易造成污染在對(duì)非常稀少或者非常有價(jià)值的樣品進(jìn)行研究時(shí)，污染常常不能被接受本文介紹了利用壓力循環(huán)技術(shù)(PCT)和傳統(tǒng)研磨技術(shù)兩種不同的方法從玉米芽葡萄種子和葡萄皮中提取RNA，并對(duì)這兩種方法進(jìn)行了比較 SPP超高壓循環(huán)樣品制備系統(tǒng)