The Q Exactive MS is well suited for targeted quantification providing two powerful HR/AM approaches, msx tSIM and tHCD. Both approaches demonstrate high selectivity, high sensitivity and high throughput due to several hardware and software innovations. ? For the msx tSIM approach, high mass accuracy (< 5 ppm) and high resolution (140K) ensures accurate identification of target ions in the presence of complex background, while spectrum multiplexing dramatically increases throughput without sacrificing resolution. ? A LOD of 10 amol and 4-order linear dynamic range was obtained for most peptides with msx tSIM method in the presence of medium complex background. ? Different from triple-quadrupole-based SRM, all fragment ions are simultaneously detected with high mass accuracy and high resolution using the tHCD approach. The HR/AM of fragment ions provides unmatched selectivity. The simultaneous detection of all fragment ions allows more efficient samp領(lǐng) of target peptides and results in improved sensitivity. A scan speed of 12 Hz at a resolution of 17,500 ensures high throughput. ? A LOD of 50 amol and 3-4 orders linear dynamic range was obtained for most peptides with tHCD method in the presence of strong complex background. 賽默飛Q Exactive Orbitrap LCMSMS系統(tǒng) 賽默飛TSQ Endura三重四極桿質(zhì)譜儀 賽默飛TSQ Quantiva 三重四極桿質(zhì)譜儀 賽默飛Q Exactive Focus LCMSMS 系統(tǒng) 賽默飛LTQ Orbitrap XL組合式質(zhì)譜儀