色譜柱 : TSKgel SuperSW series 流動相 : 20mmol/L Phosphate buffer + 0.3mol/L NaCl (pH7.0) 樣品 : MW-MARKER PROTEINS (HPLC), cat:46804000, ORIENTAL YEAST CO.,LTD, Japan 流速 : 0.35mL/min 檢測 : UV (280nm) 溫度 : 25C 進(jìn)樣體積 : 2L 液相色譜儀 : Agilent 1120 Compact LC (gradient auto system) 樣品組成 : 1. Glutamate dehydrogenase (yeast, M.W.:290KDa, 1.83g/L) 2. Lactate dehydrogenase (pig heart, M.W.:142KDa, 1.58g/L) 3. Enolase (yeast, M.W.:67KDa, 1.35g/L) 4. Myokinase (yeast, M.W.:32KDa, 1.31g/L) 5. Cytochrome C (horse heart, M.W.:12.4KDa, 1.25g/L)